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In pathological diagnosis and forensic investigations, histological staining is a frequently used medical procedure. Fixation, processing, embedding, sectioning, and staining is the five main steps in the histological staining procedure. Staining is used to draw attention to significant tissue characteristics and to improve tissue contrast. There are a number of different staining techniques that are utilized for specific cells and components. A typical medical procedure for locating sick, tumorous, or other pathological cells is staining, which involves applying a dye color to the rear and anterior boundary of sample tissues. Staining is used in biological research to identify cells, nucleic acids, proteins, and gel electrophoresis to facilitate microscopic study. Differential staining, double staining, and other multiple staining techniques may be employed under certain circumstances.