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Cytokines are small peptides or glycoproteins synthesized and secreted by a variety of tissue cells (mainly immune cells). Cytokines can mediate the interaction between cells, and have a variety of biological functions, such as regulating cell growth, differentiation, maturation, regulating immune response , participating in inflammation, etc. Signaling pathway refers to the phenomenon that when a certain reaction is to occur in the cell, a signal is transmitted from the outside of the cell to the cell, and the cell will respond according to this signal. Cytokine and signaling pathway assay helps elucidate the mechanism of physiological activities at the molecular level, thus providing directions for disease prevention, diagnosis and treatment.