Dark Field Microscopy

Dark field microscopy is a high-contrast microscopy technique used to observe living, unstained biological samples, such as a smear from a tissue culture or individual, water-borne, single-celled organisms. Specimens are brightly lit against a dark background to provide the contrast needed to study samples in solutions or natural aquatic environments such as pond water. It is a beneficial tool for studying biological and medical phenomena that cannot be easily separated from their immediate surroundings.