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Enzyme-linked immunosorbent assay (ELISA) is the most widely used technology in enzyme immunoassay. The principle is to bind the known antigen or antibody to the surface of the solid phase carrier, and utilize the specific binding of the antigen and antibody and the color reaction that occurs when the enzyme labeled on the antibody / antigen catalyzes the specific substrate to achieve target detection. ELISA is suitable for the detection of small molecule antigen, with the advantages of simple operation, rapid detection and accurate results, which is often used in clinical diagnosis, food safety, animal husbandry detection and other fields.