Lectin Affinity Chromatography Technology

Lectin Affinity Chromatography is a chromatographic technique that exploits the specific binding ability of lectins to carbohydrates, particularly glycan structures found on glycoproteins. Lectins are proteins or glycoproteins that possess at least one non-catalytic domain capable of binding to specific sugar moieties. This characteristic makes lectins an invaluable tool in the isolation and analysis of glycoproteins, as glycosylation is a critical post-translational modification that influences protein structure and function. Lectin affinity chromatography is the most frequently used specific purification procedure for glycoproteins. It can be used for glycoprotein analysis and profiling, glycomic studies, glycoengineering and biopharmaceuticals, cell surface glycoprotein studies