Microscale Thermophoresis (MST) Technology

MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge, or size. It records the changes in fluorescence of a target molecule as a function of temperature and the concentration of the cognate ligand molecule. Microscale thermophoresis assays are routinely used to investigate protein–protein interactions and protein−ligand interactions and to guide the discovery of new modulators. It can determine the important parameters in a rapid and precise manner, with free choice of buffers or biological liquids, no need for sample immobilization, and very low sample consumption.