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Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the size, concentration, zeta potential and fluorescence of particles in liquid suspension. A microscopic setup allows real-time visualization of particles below the diffraction limit of conventional microscopes, in both scatter and fluorescence mode. A broad range of applications from nano-sized metallic particles to polymers and biological nanoparticles such as viruses or extracellular vesicles makes NTA a versatile method for sizing – independent of the refractive index of the particle material. This technology can be used for exosomes, microvesicles and extracellular vesicles characterization, viral vaccine research and development, drug delivery systems development, protein aggregation studies and nanotoxicology.