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Nuclear magnetic resonance (NMR) spectroscopy is a branch of spectroscopy that can be used to determine the number, type, and relative positions of components in mixtures. Because of its characteristics of high throughput, high sensitivity and high stability, especially its advantages of "fingerprint", non-destructive and non-biased detection of metabolite components, NMRS has become one of the most commonly used analytical detection technologies in metabolomics. In biology, NMR can be used to study large molecules, such as proteins, lipids, and nucleic acids. In chemistry, NMR is widely used for qualitative analysis, quantitative analysis, monitoring reactions, identification of structures and determination of purity.