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Paper chromatography is a technique in analytical chemistry, which separates dissolved chemical substances based on their different rates of migration across sheets of paper. Paper Chromatography has many benefits: Simple, rapid, only need a minimal amount of quantitative material, and It is less expensive than other chromatography methods. There are various applications of paper chromatography. For example, It can be used for checking the purity of pharmaceuticals, inspecting cosmetics, and detecting the contaminants in drinks and foods. Paper chromatography has become standard practice for the separation of complex mixtures of amino acids, peptides, carbohydrates, steroids, purines, and a long list of simple organic compounds. Inorganic ions can also readily be separated on paper.