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Polymerasechain reaction (PCR) is an in vitro synthesis technique that amplifies large amounts of specific DNA fragments. The principle is to copy the complementary daughter strand DNA with the template DNA of the parent strand by denaturation, annealing and extension under the catalysis of DNA polymerase, using the parent strand DNA as the template and the specific primer as the extension starting point. PCR technology is widely used in basic research, medical diagnosis, forensic medicine, agricultural science and other fields, and has great significance for the development and progress of life science.