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Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. TLC is based on the classic chromatography principle where mixture components are separated between a fixed stationary phase and a liquid mobile phase by differential affinities between the two phases. It is one of the easiest and most versatile methods of doing this because of its low cost, simplicity, quick development time, high sensitivity, and good reproducibility. TLC is widely used by many industries and research fields, including pharmaceuticals, clinical testing, environmental toxicology, water, food and pesticide analysis, and cosmetics.