Cell Counting by Flow Cytometry (CAT#: STEM-CBT-0072-WXH)

A known amount of a fluid containing a known concentration of labelled synthetic beads is added to a known volume of the sample, and the cells of the population or populations of interest are suitably labelled in known manner with labels distinguishable from those of the synthetic beads. The number of cells of the population or populations of interest are counted, as well as the number of labelled synthetic beads passing through the cytometer in the same period. Knowing the relative numbers of cells of a given population and the synthetic beads, and the concentration of the synthetic beads (e.g. number of synthetic beads per ml), the absolute number of cells of the given population for may be determined, and similar calculations simultaneously or sequentially may be made.
As the particles or cells pass through the laser beam, they refract and reflect the light. This refraction and reflection signal is recorded by the detector. For each cell that passes through, a peak is generated, and finally the number of peaks is recorded and the count is made

• Quantifying cell populations and disease progression, including studies of stem cells, HIV/AIDS, and leukemia.
• Monitoring the disease status of HIV-infected patients.
• Enumerating residual white blood cells in leukoreduced blood products.
• Assessing immunodeficiency in a variety of situations.

1.Process cells as needed before counting
2.Add bead suspension to the sample
3.Run the sample on the flow cytometer
4.Acquire the samples
5.Calculate cell concentration

Absolute Counting Beads