Cell Recovery

Cells resuscitation should be rapidly melted, which can ensure that extracellular crystals melt in a very short time, to avoid the slow melting of water into the cell to form intracellular recrystallization caused by cell damage.

• Preparation

1. Preheat the water bath to 37℃ and set aside.
2. Prepare a 15mL centrifuge tube and add an appropriate amount of medium to it for use.

• Cell thawing

1. Remove the cell cryopreserved tube from -80℃ refrigerator or liquid nitrogen, put it into PE gloves, and quickly put it into the water bath.
2. Shake the freezing tube vigorously to melt it in 1 minute.

• Cell vaccination

1. Wipe the water stains with paper towels and transfer them to the biosafety cabinet. The cell suspension was aspirated with a pipette gun and added slowly in drops to the centrifuge tube prepared.
2. Centrifuge at 1200rpm for 3 min.
3. Discard the supernatant, resuspend the cells with fresh medium, and inoculate them into a new sterile culture vessel.

Fig. 1 Schematic diagram of cell resuscitation operation.

After cell recovery, it can enter the cell cycle and regain cell-type-specific biological functions.

Laboratory Centrifuges

A centrifuge is any device that applies a sustained centrifugal force—that is, a force due to rotation. The widest use of centrifuges is for the concentration and purification of materials in suspension or dissolved in fluids. Suspended particles denser than the suspending liquid tend to migrate toward the periphery, while those less dense move toward the centre.

Liquid Nitrogen Container

Liquid nitrogen storage equipment is used to store biologic, genomic, and diagnostic samples in liquid nitrogen. Liquid nitrogen tanks can generally be divided into storage and transport tanks. The storage tank is mainly used for static storage of indoor liquid nitrogen, and it is not suitable for long-distance transportation under working conditions. The transportation tank has a special shockproof design to meet the transportation conditions.

Water Baths

A water bath is laboratory equipment made from a container filled with heated water. It is used to incubate samples in water at a constant temperature over a long period of time. Most water baths have a digital or an analogue interface to allow users to set a desired temperature. A laboratory water bath is a preferred heat source for heating flammable chemicals instead of an open flame to prevent ignition.

Laboratory Refrigeration

Laboratory refrigerators are designed with special features and options for use in the laboratory, including a range of refrigerator and freezer accessories. The primary function of a laboratory refrigerator is to maintain a defined, internal storage temperature in order to provide secure storage and protection of temperature-sensitive products, samples, specimens, chemicals, drugs, solutions, and other substances.

Ultra-clean Bench

The ultra-clean workbench is a kind of unidirectional flow air purification equipment that provides a local dust-free and sterile working environment. Most of the ultra-clean workbenches are 100 grades, which are mainly divided into two categories, one is horizontal laminar flow, and the other is vertical laminar flow. It can be used in pharmaceutical, medical and health, university scientific research laboratories and other fields.

Laboratory Incubators

An incubator is a device used to grow and maintain microbiological cultures or cell cultures. It is made up of a chamber with a regulated temperature. An incubator maintains optimal temperature, humidity, and other conditions such as the CO₂ and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology, microbiology, and molecular biology.

Biosafety Cabinets

Biosafety Cabinets are enclosed workspaces with a ventilated hood that is designed to contain pathogenic microorganisms during microbiological processes. Their primary purpose is to protect the laboratory personnel and the environment from the pathogenic microorganism as aerosols might be formed during the processing of such microorganisms. They are only used for certain risk group organisms and for processes that might result in aerosol formation.

STEMart provides you with a variety of cell culture equipment or consumables to meet your various R&D and application needs. If you have any questions or requirements for primary cell recovery, please feel free to contact us.