CellThru 10-ml Disposable Columns, 20 Columns , 635513, Takara Bio Inc (CAT#: STEM-C-3818-LGZ)

TALON CellThru is a novel IMAC resin for the purification of his-tagged proteins from crude cell lysates, sonication and fermentation harvests. This resin uses the same proprietary ligands as our TALON metal affinity resins, but has larger beads (300-500 microns) that allow cell debris to flow through without centrifugation. TALON CellThru resin captures His-tagged proteins directly from crude lysates, a rapid step that minimizes protein degradation and yields higher yields of purified protein than traditional strategies.

TALON CellThru Resin is ideal for purification of membrane-bound proteins or multiprotein complexes that are typically pelleted with cell debris during lysis clarification steps and cannot be bound to the resin. Because crude, unclarified lysate can be applied directly to the resin, you can skip the centrifugation step, allowing membrane-bound proteins and multiprotein complexes to remain in the lysate, where they can bind to the resin.

The resin consists of large agarose beads that can be packed into standard chromatography columns to prevent column clogging. Particulate matter flows between the beads, while soluble protein products are captured by ion-exchange functional groups attached to the beads. This resin is well suited for extended bed chromatography to recover higher amounts of protein and is recommended for applications such as high-throughput purification of his-tagged proteins from crude extracts. We also offer CellThru 10ml disposable columns (90-130 micron filter pore size) that allow cell debris to flow through easily.

Size: 20 Columns

Purification from crude cell lysate
Ideal for purification of membrane-bound protein or multiprotein complexes
No copurification of proteins
Low metal ion leakage
Fast & easy protocol

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