Characterisation of membrane mimetics by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0410-WXH)

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Characterisation of membrane mimetics.
Study of the structure and features of lipid membranes.
Drug Discovery.

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Formation of a fatty acid monolayer on an amine
modified sensor chip surface
4. Production of hybrid bilayer membranes (HBM)
5. The chip is inserted into the
instrument and the fluidic system is started
6. Result analysis

• DPI biosensor
• DPI sensor chip