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Characterization of amino acids by Raman Spectroscopy (CAT#: STEM-ST-0101-WXH)

Introduction

Amino acids are the basic “building blocks” that combine to form proteins. In every species, proteins are constructed from the same set of twenty amino acids. As well as forming proteins, enzymes and other body tissue they are also found throughout the body participating in a wide variety of chemical reactions, and are vital in basic energy production cycles, energy transfer and muscle activity. In the higher organisms including humans, amino acids are classified as either “essential” amino acids (which must be consumed in the diet), or “non-essential” amino acids (which can be made by the body)




Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope
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