Characterization of antibody-polyol interactions by static light scattering by Static light scattering (SLS) (CAT#: STEM-MB-0583-WXH)

Introduction

Polyols are alcohols with more than one hydroxyl group and they constitute one of the main raw materials for manufacturing polyurethane. The addition of polyol osmolytes has been shown to prevent aggregation, retain activity, inhibit chemical degradation, enhance the refolding of proteins, and increase the thermal stability of proteins. It has been shown that the more hydroxyl groups on the carbon chain of the polyol, the greater the thermodynamic stability that can be induced.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Static light scattering is a technique in physical chemistry that measures the intensity of the scattered light to obtain the average molecular weight Mw of a macromolecule like a polymer or a protein in solution. Measurement of the scattering intensity at many angles allows calculation of the root mean square radius, also called the radius of gyration Rg. By measuring the scattering intensity for many samples of various concentrations, the second virial coefficient, A2, can be calculated.

Applications

The main applications of static light scattering is molecular mass determination of macromolecules, such as proteins and polymers, as it is possible to measure the molecular mass of proteins without any assumption about their shape.

Procedure

1. Sample preparation
2. Measurement by SLS instrument
3. Data analysis

Materials

• Right-Angle Light Scattering (RALS) Detector
• Low-Angle Light Scattering (LALS) Detector
• Hybrid RALS/LALS Detector
• Multi-Angle Light Scattering (MALS) Detector

Other recommended products

Analysis of Lipopolysaccharide Aggregation by Static light scattering (SLS) (CAT#: STEM-MB-0588-WXH)
Molecular mass analysis of Carrageenans by Static light scattering (SLS) (CAT#: STEM-MB-0561-WXH)
Molecular mass analysis of plasmid DNA by Static light scattering (SLS) (CAT#: STEM-MB-0570-WXH)
Molecular mass analysis of red blood cells by Static light scattering (SLS) (CAT#: STEM-MB-0586-WXH)
Molecular mass analysis of pullulan by Static light scattering (SLS) (CAT#: STEM-MB-0589-WXH)
Virus quantification by Static light scattering (SLS) (CAT#: STEM-MB-0592-WXH)
Molecular mass analysis of OmpF porin by Static light scattering (SLS) (CAT#: STEM-MB-0572-WXH)
Molecular mass analysis of aggregation of jack bean urease by Static light scattering (SLS) (CAT#: STEM-MB-0575-WXH)