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Gold nanoparticles (AuNPs) are small gold particles with a diameter of 1 to 100 nm which, once dispersed in water, are also known as colloidal gold. Functionalized gold nanoparticles with controlled geometrical and optical properties are the subject of intensive studies and biomedical applications, including genomics, biosensorics, immunoassays, clinical chemistry, laser phototherapy of cancer cells and tumors, the targeted delivery of drugs, DNA and antigens, optical bioimaging and the monitoring of cells and tissues with the use of state-of-the-art detection systems.