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Conjugation Services (CAT#: STEM-MB-0246-WXH)

Introduction

Conjugation is the process of covalently linking two molecules/polymers or biomolecules/biopolymers together. Conjugation of small molecule therapeutics to polymers can enhance efficacy by improving pharmacokinetic and pharmacodynamic properties by reducing plasma protein binding, reducing toxicity, or by improving stability.
The services provides convenient custom Conjugation Services that leverage expertise and knowledge in reagent manufacturing for conjugating biomolecules with either biotin or SULFO-TAG for use on platform. Biotin-conjugated material may be used as either capture or detection reagent in assays, whereas SULFO-TAG-conjugated molecules serve as detectors. The standard Conjugation Service is available for antibodies, proteins, peptides, and oligonucleotid.




Applications

A tool for Drug Delivery, Targeted Drug Delivery, Synergistic Drug and Gene Delivery, or Diagnostic applications
Immunoassays (ELISAs and Westerns being the most popular applications)
Affinity chromatography
Pull-down assays, supramolecular construction
Targeting of cancer cells for drug delivery

Procedure

Take Biotinylation of Antibodies as example:
Preparation of antibody
1.Dialyze or exchange over a column the antibody in "B Reaction Buffer".
2.Measure the antibody concentration after buffer equilibration. If the antibody concentration is less than 1 mg/ml, the conjugation will probably be sub-optimal. If necessary, dilute the antibody to a concentration of 4 mg/ml.
Covalent conjugation
3.Dissolve biotin in anhydrous DMSO immediately before use.
4.Add biotin and mix immediately.
5.Wrap the tube in foil; incubate and rotate at room temperature.
6.Remove the unreacted biotin and exchange the antibody into "Storage Buffer" by gel filtration or dialysis.
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