Conventional PCR (CPCR) (CAT#: STEM-MB-0184-WXH)

The PCR technique is based on the enzymatic replication of DNA. In PCR, a short segment of DNA is amplified using primer mediated enzymes. DNA Polymerase synthesises new strands of DNA complementary to the template DNA. The DNA polymerase can add a nucleotide to the pre-existing 3'-OH group only.

• DNA sequencing and gene reproduction & cloning.
• Cellular cloning.
• Identification of genetic fingerprints.
• Discovering the possibility of transmitting genetic diseases between potential parents and determining whether the diseases will or will not be passed to their next generation of children.
• DNA-based phylogeny, or functional analysis of genes.
• The diagnosis of hereditary diseases.
• The detection and diagnosis of infectious diseases.

1.Initialization
2.Denaturation
3.Annealing
4.Extension/elongation

What are the 5 key basic reagents used in PCR?
Generally, a complete polymerase chain reaction (PCR) requires five basic reagents, including DNA template, forward and reverse primers, DNA polymerase, deoxynucleotide triphosphates (dNTPs) and reaction buffer.