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This service provides a capillary electrophoresis (CE) method for creatinine determination in serum. Creatinine in human serum is separated in a fused-silica capillary with H3PO4 (75 mmol/L, pH 2.5) as BGE, followed by UV detection at 200 nm. Capillary electrophoresis, rapid and inexpensive, is a promising alternative to high-performance liquid chromatography (HPLC) for creatinine determination in human serum.