Cryo-Electron Microscopy (Cryo-EM) (CAT#: STEM-PET-0018-WXH)

Introduction

Electron Microscopy (EM) has become an extremely popular method for the ultrastructural study of macromolecules, cells, and tissues. An aqueous biological sample is frozen rapidly and irradiated with a beam of electrons. A detector senses how the electrons are scattered, and a computer reconstructs the 3D-shape of the molecule.

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Principle Applications Procedure Materials Notes Related Products

Principle

It works on the principle of imaging the radiation-sensitive samples by a (TEM) under low temperature conditions.

Applications

Determine the structure of biological macromolecules and macromolecular super complexes. The most typical examples are spliceosomes and chromosomes.

Procedure

(Take protein as an example)
1. Purified Protein
2. Sample preparation
3. EM imaging and data processing
4. Model building and refinement

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