Cryo-Electron Microscopy (Cryo-EM) (CAT#: STEM-PET-0018-WXH)

Introduction

Electron Microscopy (EM) has become an extremely popular method for the ultrastructural study of macromolecules, cells, and tissues. An aqueous biological sample is frozen rapidly and irradiated with a beam of electrons. A detector senses how the electrons are scattered, and a computer reconstructs the 3D-shape of the molecule.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

It works on the principle of imaging the radiation-sensitive samples by a (TEM) under low temperature conditions.

Applications

Determine the structure of biological macromolecules and macromolecular super complexes. The most typical examples are spliceosomes and chromosomes.

Procedure

(Take protein as an example)
1. Purified Protein
2. Sample preparation
3. EM imaging and data processing
4. Model building and refinement

Other recommended products

Morphology study of the Cementoenamel Junction in Permanent Teeth of Dogs by scanning electron microscope technology (CAT#: STEM-MIT-0069-LJX)
Study of root surface following mini-implant anchorage for maxillary incisors intrusion by scanning electron microscope technology (CAT#: STEM-MIT-0088-LJX)
Observation of primary tooth dentin following final irrigation with 95% ethanol by scanning electron microscope technology (CAT#: STEM-MIT-0055-LJX)
Observation of the external morphology of Dermatophagoides farinae at different developmental stages by scanning electron microscope technology (CAT#: STEM-MIT-0052-LJX)
Morphological studies of the lingual papillae of the tongue of the goat (Capra hircus) by scanning electron microscope technology (CAT#: STEM-MIT-0060-LJX)
Histological studies of the buccal cavity of a phytoplanktivorous small freshwater fish, Amblypharyngodon mola by scanning electron microscope technology (CAT#: STEM-MIT-0048-LJX)
Detection of HIV-p24 antigen in body fluids by immunotrapping on Staphylococcus aureus (Cowan 1) bacteria, gold immunolabelling and backscattered electron analysis in a scanning electron microscope (CAT#: STEM-MIT-0059-LJX)
Study of taxonomic implications of macro and micromorphological characters in the genus Brachythecium (Brachytheciaceae, Bryopsida) from the Western Himalayas by light and scanning electron microscope technology (CAT#: STEM-MIT-0073-LJX)