CX3C Subfamily Detection Service (CAT#: STEM-MB-0262-WXH)

CX3C chemokines have a CX3C receptor, CX3CR1, which is the receptor for fractalkine. Through in-depth research on it, scientists found that its downstream signaling mechanism is similar to other subgroups, and it is through the binding of chemokines to chemokine receptors on the surface of target cells, which then activates the intracellular coupling of G protein to cause G Protein subunits are released. Free G protein subunits activate phospholipase C (PLC). The activated phospholipase C cleaves phosphatidylinositol (4,5) -diphosphate (PIP2) into inositol triphosphate (IP3) and diacylglycerol ( DAG). Among them, IP3 can cause changes in intracellular calcium ions and subsequent signal transduction, while DAG can activate protein kinase C (PKC). These events can promote subsequent cascades of related intracellular signals that ultimately cause the adhesion and migration of leukocytes.

Stimulate the migration of cells.
Analyze the function of chemokines, from intracellular transport to signal transduction activities.
Study the relationship between chemokines and the mechanism of occurrence and metastasis of various diseases. For example, it plays a role in the production and differentiation of immune cells and bone marrow hematopoietic cells, development regulation and immune response regulation.
Develop new anti-inflammatory drugs based on the physiological functions of chemokines.

1. Process samples.
2. CX3C Subfamily detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types - Blood, serum, plasma, cell culture supernatant, cell lysate, cell culture medium, tissue homogenate, urine, tumor, etc.