CXC Subfamily Detection Service (CAT#: STEM-MB-0259-WXH)

The signals generated by the binding of CXC chemokines to receptors need to be transduced with the help of G proteins near the intracellular receptors. Generally, G proteins exist as heterotrimers, which are composed of three different subunits: Gα, Gβ, and Gγ. At this time, the GDP molecule is bound to the G protein subunit, and the G protein is in an inactive state. When the receptor is activated, G protein is recruited to the intracellular receptor, thereby replaced GDP by GTP molecules and dissociating different G protein subunits. The Gβ subunit activates a phospholipase C (PLC) on the cell membrane, which can cleave phosphatidylinositol (4,5) -bisphosphate (PIP2) to form two second messenger molecules, called inositol Triphosphate (IP3) and diacylglycerol (DAG); DAG activates another enzyme called protein kinase C (PKC), and IP3 triggers the release of calcium from the endoplasmic reticulum. These events promote many signal cascades and affect cellular responses. In addition, the combination of CXC and CXCR also activates NF-κB, Camp / PKA, and other pathways, which together with the aforementioned pathways regulate cell adhesion, polarization, and directional movement.

The main role of chemokines is to induce directional movement of cells.
Analyze the function of chemokines, from intracellular transport to signal transduction activities.
Study the relationship between chemokines and the mechanism of occurrence and metastasis of various diseases. For example, it plays a role in the production and differentiation of immune cells and bone marrow hematopoietic cells, development regulation and immune response regulation.
Develop new anti-inflammatory drugs based on the physiological functions of chemokines.

1. Process samples.
2. CXC Subfamily detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types - Blood, serum, plasma, cell culture supernatant, cell lysate, cell culture medium, tissue homogenate, urine, tumor, etc.