Detection of bacterial spores by Coherent Anti-Stokes Raman Spectroscopy (CARS) (CAT#: STEM-ST-0037-WXH)

Introduction

Bacterial spores are the most dormant form of bacteria since they exhibit minimal metabolism and respiration, as well as reduced enzyme production. Typically, Gram-positive bacteria are best known for producing intracellular spores called endospores as a survival mechanism.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Coherent Anti-Stokes Raman Spectroscopy (CARS) is a nonlinear Raman spectroscopy technique that uses two very strong collinear lasers to irradiate a sample. The frequency is usually kept constant, with the second laser tuned so that the frequency difference between the two lasers equals the frequency of a Raman-active mode of interest.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

Other recommended products

Assessmnet of the thyroid stimulating Hormone (TSH) in blood by Raman spectroscopy (CAT#: STEM-ST-0039-WXH)
Study of saturated fatty acids by Raman Spectroscopy (CAT#: STEM-ST-0081-WXH)
Analysis of Fruits and Vegetables by Raman Spectroscopy (CAT#: STEM-ST-0089-WXH)
Analysis of Proteinase by Raman Spectroscopy (CAT#: STEM-ST-0017-WXH)
Characterization of antimicrobial photodynamic therapy against Staphylococcus epidermidis by Raman Spectroscopy (CAT#: STEM-ST-0022-WXH)
Study of human coronary arteries by Raman Spectroscopy (CAT#: STEM-ST-0068-WXH)
Study of Heme proteins by Raman Spectroscopy (CAT#: STEM-ST-0021-WXH)
Study of the enzyme eosinophil peroxidase in living immune cells by Raman Spectroscopy (CAT#: STEM-ST-0078-WXH)