Detection of EGFR Gene Mutation in Lung Cancer by Mutant-Enriched Polymerase Chain Reaction Assay (CAT#: STEM-MT-0008-LGZ)

Introduction

Epidermal growth factor receptor (EGFR) gene mutations have been reported to be present in non-small cell lung cancer (NSCLC) and are associated with tumor responsiveness to EGFR tyrosine kinase inhibitors, suggesting its usefulness as a biomarker. Since clinical samples contain both tumor cells and normal cells or genes, high-sensitivity assays to detect mutations are critical for clinical applications.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Lung Cancer

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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