Detection of IDH1 Mutation in cfDNA and Tissue of Adult Diffuse Glioma by RT-qPCR (CAT#: STEM-MT-0023-LGZ)

Introduction

The World Health Organization (WHO) classification of central nervous system (CNS) tumors requires detection of isocitrate dehydrogenase (IDH) 1/2 gene mutations in patients with adult-type diffuse glioma (ADG) for better management. In clinical practice, the detection of IDH1 is mainly achieved by IDH1-r132-specific immunohistochemistry (IHC), which has a sensitivity of 80% and a specificity of 100%. However, in some cases, nonspecific background staining or regional heterogeneity of IDH1 protein expression may warrant confirmatory genetic analysis. IDH1/2 mutation detection requires reliable detection methods.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Central Nervous System (CNS) Tumors

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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