Detection of Ile491Phe rpoB Mutation of Mycobacterium Tuberculosis by RT-qPCR (CAT#: STEM-MT-0035-LGZ)

Introduction

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is a major global public health problem. Tuberculosis control is complicated by drug resistance, especially to first-line rifampicin (RIF) and isoniazid (INH), collectively known as multidrug resistance (MDR-TB). To acquire resistance to anti-TB drugs, M. tuberculosis drug targets or activators are frequently mutated.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Detection of genetic mutations.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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