Detection of K-ras Mutation in Colorectal Cancer by RT-qPCR (CAT#: STEM-MT-0033-LGZ)

Introduction

An important step in colorectal cancer progression involves inducing activation mutations in the proto-oncogene K-ras. The K-ras gene encodes a 21 kDa RAS protein and is a member of the highly conserved gtpase family involved in signal transduction processes. The oncogenic form of K-ras is a constitutive activator protein derived from a point mutation that results in a reduction in intrinsic Gtase activity, a process that is required for the conversion of RAS protein to an inactivated state. Thus, changes affecting K-ras oncogenes lead to increased cell proliferation.