Detection of Multiple K-ras Codon 12 Mutations in Cholangiocarcinomas by RT-qPCR (CAT#: STEM-MT-0031-LGZ)

Introduction

Cholangiocarcinoma is a common complication of primary sclerosing cholangitis (PSC). K-ras mutations were located at codon 12 in most tumors (n=9).

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Cholangiocarcinomas

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Red Sea Bream Iridovirus (RSIV) by Real-Time PCR Method (CAT#: STEM-MB-3467-LGZ)
Analysis of FAM177B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0943-LGZ)
Analysis of Histoplasma Capsulatum Var. Farciminosum by Real-Time PCR Method (CAT#: STEM-MB-3715-LGZ)
Analysis of Enterovirus (EV) - D by Real-Time PCR Method (CAT#: STEM-MB-4021-LGZ)
Analysis of CHRNA10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2230-LGZ)
Analysis of Staphylococcus Nepalensis by Real-Time PCR Method (CAT#: STEM-MB-3382-LGZ)
Analysis of TGFBR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1934-LGZ)
Analysis of OR56A1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2356-LGZ)