Detection of PIK3CA Mutations by RT-qPCR (CAT#: STEM-MT-0014-LGZ)

Introduction

PIK3CA mutations appear to be the most common driver mutations in breast cancer, with H1047R and E545K being the most common, accounting for approximately 60% of all PIK3CA mutations, with good therapeutic implications. Given the low sensitivity and high cost of current genotyping methods, we offer a fast, simple and inexpensive assay for PIK3CA H1047R and E545K mutation screening in clinical materials.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Breast cancer

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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