Detection of unidentified chromosome abnormalities in human neuroblastoma by spectral karyotyping (SKY) (CAT#: STEM-ST-0384-WXH)

Introduction

Neuroblastoma (NB) is a type of cancer that forms in certain types of nerve tissue. It most frequently starts from one of the adrenal glands but can also develop in the head, neck, chest, abdomen, or spine. Symptoms may include bone pain, a lump in the abdomen, neck, or chest, or a painless bluish lump under the skin.

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Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

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