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Royal jelly (RJ) is a whitish cream essential for the growth of queen honeybees. RJ, consisting mainly of water, proteins, carbohydrates, fatty acids and vitamins, has been used worldwide in dietary supplements, medical products, and cosmetics due to the belief that it exerts similar effects on human beings as it does on honeybees. The quality of RJ has been based on the content of trans-10-hydroxy-2-decenoic acid (10-HDA). This service provides a method for determination of 10-hydroxy-2-decenoic acid (10-HDA) and free amino acids in different royal jelly based dietary supplement samples using a purpose-made dual-channeled capillary electrophoresis (CE) system with capacitively coupled contactless conductivity detection (C4D).