Determination of Chondroitin Sulfate A with Crystal Violet by Resonance Rayleigh Scattering Technique (CAT#: STEM-ST-0056-YJL)

Introduction

Chondroitin sulfate (CS) is a glycosaminoglycan consisting of repeating uronic acid (D-glucuronic acid or L-iduronic acid), N-acetylgalactosamine (GalNAc) sulfate disaccharide units [-UroA(β-1,3)-Gal NAcS(β1,4)]n. According to the variety of uronic acid or N-acetylgalactosamine sulfate, CS can be divided into several kinds, such as chondroitin sulfate A (CSA), chondroitin sulfate B (CSB), chondroitin sulfate C (CSC), etc, in which CSA and CSC are the major ingredients in almost any animal species and tissue.
Crystal violet (CV) is a basic triphenylmethan dye, which exist as a cationic species in near neutral solution. The intensity of RRS can be greatly enhanced by the interaction between CSA and CV and that a new RRS spectrum is produced owing to the formation of CSA-CV supramolecular complex by electrostatic gravitation and hydrophobic force.