Determination of human insulin and its analogues in human blood by liquid chromatography coupled to ion mobility mass spectrometry (LC-IM-MS) (CAT#: STEM-ST-0164-LJX)

Introduction

The qualitative and quantitative determination of insulin from human blood samples is an emerging topic in doping controls as well as in other related disciplines (e.g. forensics). Beside the therapeutic use, insulin represents a prohibited, performance enhancing substance in sports drug testing. In both cases accurate, sensitive, specific, and unambiguous determination of the target peptide is of the utmost importance. Liquid chromatography combined with ion mobility mass spectrometry (LC-IM-MS) can be used to identify insulin in the blood.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale.

Applications

For studying the gas phase ion structure
For detecting the chemical warfare agents and explosives
For the analysis of proteins, peptides, drug-like molecules and nano particles
For monitoring isomeric reaction intermediates and probe their kinetics
For proteomics and pharmaceutical analysis

Procedure

1. Add sample
2. The ions in the sample are separated in the ion mobility spectrometer
3. The separated ions are introduced into the mass analyzer for detection
4. Store the detection results

Materials

• Sample Type:
Human blood

Notes

1. Ion mobility spectrometry is also a very fast technique, making it suitable for high-throughput applications. The entire analysis can be completed in just a few minutes.
2. The method is extremely sensitive and able to detect trace amounts of contaminants that other spectrometry methods would miss.
3. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.

Other recommended products

Negative Atmospheric Pressure Chemical Ionization of Chlorinated Hydrocarbons by Ion Mobility Mass Spectrometry (CAT#: STEM-ST-0131-LJX)
Isomer Separation of Iron Oxide Cluster Cations by Ion Mobility Mass Spectrometry (CAT#: STEM-ST-0171-LJX)
Analysis of Carbohydrate Chemistry and Glycobiology by Ion Mobility-Mass Spectrometry (CAT#: STEM-ST-0081-LJX)
Gas-phase metalloprotein complexes interrogated by ion mobility-mass spectrometry (CAT#: STEM-ST-0165-LJX)
Analysis of multiple classes of steroid hormone isomers in a mixture by liquid chromatography-ion mobility spectrometry-mass spectrometry (CAT#: STEM-ST-0102-LJX)
Analysis of a rotary ATPase by Ion mobility-mass spectrometry for revealing ATP-induced reduction in conformational flexibility (CAT#: STEM-ST-0107-LJX)
Resolving Disulfide Structural Isoforms of IgG2 Monoclonal Antibodies by Ion Mobility Mass Spectrometry (CAT#: STEM-ST-0151-LJX)
Analyzing slowly exchanging protein conformations by ion mobility mass spectrometry (CAT#: STEM-ST-0129-LJX)