Determination of Melting Point of Flavonoids by Capillary Method (CAT#: STEM-PPA-0053-YJL)

Introduction

Flavonoids are among the most important plant metabolites. Due to their potential benefits, there is a considerable interest in this natural product. In genus Citrus, some plants have not yet been much exploited in Brazil, as in the case of grapefruit (Citrus maxima), whose main flavonoids are naringin and their aglycone naringenin. The physico-chemical characteristics are important pre-requisites of reference chemical in future studies.

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Principle Applications Procedure Materials Notes Related Products

Principle

Melting point is a characteristic property of solid crystalline substance. It is the temperature at which the solid phase changes to the liquid phase. This phenomenon occurs when the substance is heated.
The melting point measurement is usually performed in thin glass capillary tubes with an internal diameter of 1 mm and a wall thickness of 0.1 – 0.2 mm. A finely-ground sample is placed in the capillary tube to a filling level of 2 – 3 mm and introduced in a heated stand (liquid bath or metal block) in close proximity to a high accuracy thermometer. The temperature in the heating stand is ramped at a user-programmable fixed rate. The melting process is visually inspected to determine the melting point of the sample.

Applications

Chemical industry; Cosmetic/pharmaceutical industry

Procedure

1. Seal capillary.
2. Fill the sample.
3. Install the instrument.
4. Determine the melting point.

Materials

• Sample Type: crystalline compounds

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