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Determination of Melting Point of Flavonoids from Mango by Capillary Method (CAT#: STEM-PPA-0058-YJL)

Introduction

Flavonoids are a major class of oxygen-containing heterocyclic natural products that are widespread in green plants. Generally, they are found as plant pigments in a broad range of fruits and vegetables. These are C15 compounds composed of two aromatic rings linked through a three-carbon bridge with a carbonyl functional group located at one end of the bridge. Flavonoids have been recognized as having a protective effect in plants against microbial invasion by plant pathogens.
Mango (Mangifera indica L.) is an economically important tropical fruit found throughout the world. Mango contains various classes of polyphenols, carotenoids, and ascorbic acid, which demonstrate different health-promoting properties, mainly from their antioxidant activities.




Principle

Melting point is a characteristic property of solid crystalline substance. It is the temperature at which the solid phase changes to the liquid phase. This phenomenon occurs when the substance is heated.
The melting point measurement is usually performed in thin glass capillary tubes with an internal diameter of 1 mm and a wall thickness of 0.1 – 0.2 mm. A finely-ground sample is placed in the capillary tube to a filling level of 2 – 3 mm and introduced in a heated stand (liquid bath or metal block) in close proximity to a high accuracy thermometer. The temperature in the heating stand is ramped at a user-programmable fixed rate. The melting process is visually inspected to determine the melting point of the sample.

Applications

Chemical industry; Cosmetic/pharmaceutical industry

Procedure

1. Seal capillary.
2. Fill the sample.
3. Install the instrument.
4. Determine the melting point.

Materials

• Sample Type: crystalline compounds
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