Determination of nucleic acid–ligand interactions by Circular dichroism (CD) (CAT#: STEM-MB-0609-WXH)

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Circular dichroism (CD) spectrophotometer