Diagnosis of leukemia by surface enhanced laser desorption/ionization time-of-flight mass spectrometry (CAT#: STEM-ST-0360-LJX)

Introduction

This study was purposed to find new biomarkers and to establish protein finger print model for diagnosis of leukemia. A total of 40 leukemia samples and 37 healthy control samptes were tested by surface enhance laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF- MS). The data of spectra were analyzed by bioinformatics tools like Biomarker Patterns 5.0 and discriminant analysis to establish diagnostic mode1. The results showed that 22 protein features were stably detected by protein fingerprint, The detective model combined with 3 biomarkers (m/z 4650, 8609 and 11660) could differentiate leukemia with sensitivity of 97.5% (39/40) and specificity of 91.9%(34/37). It is concluded that the detective model established by 3 protein features may be a novel method for diagnosis of leukemia.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

The surface enhanced laser desorption ionization technique belongs to laser desorption mass spectrometry (LDMS). It is different from ordinary LDMS in that the laser is not directly hit on the sample to desorption, but the sample is suspended in the matrix, the laser is hit on the matrix, the matrix absorbs and transmits the laser energy, so that the sample in the matrix desorption out. After desorption and ionization, the samples were examined in a time-flight mass spectrometer.

Applications

For protein analysis and measurement of molecular weight of complete proteins
For the diagnosis of a variety of diseases, especially cancer

Procedure

1. The surface of the protein chip is treated in a certain chemical or biochemical way (surface enhancement), so that it has the ability to bind specifically to a certain type of protein
2. The serum or protein extract is directly added to the surface of the chip, and the chip is washed after incubation. Specific proteins bind to the chip and are thus separated from the protein mixture
3. The chip then uses a "chip reader" (a kind of SELDI-TOF-MS) to obtain a mass spectrum of the protein bound to the chip
4. The SELDI protein chip system can be used to compare changes in the protein profile of any set of control samples or different disease states to identify biomarkers or disease-related targets

Materials

• Sample Type:
Serum

Notes

When operating, strictly follow the experimental steps.

Other recommended products

Analysis of phospholipids on Gold Nanoparticles by Surface Enhanced Laser Desorption Ionization Mass Spectrometry (CAT#: STEM-ST-0343-LJX)
Identifing patients with chronic obstructive pulmonary disease by surface enhanced laser desorption/ionization (SELDI) time-of-flight mass spectrometry (CAT#: STEM-ST-0345-LJX)
Identification of serum biomarkers for canine B-cell lymphoma by surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (CAT#: STEM-ST-0374-LJX)
Analysis of protein and Peptide profiling of body fluids by surface-enhanced laser desorption/ionization mass spectrometry (CAT#: STEM-ST-0341-LJX)
Protein profiling of single epidermal cell types from Arabidopsis thaliana by surface-enhanced laser desorption and ionization technology (CAT#: STEM-ST-0366-LJX)
Revealing the Significant Artifacts in Serum Obtained from Clot Activator–Containing Collection Devices by Surface-Enhanced Laser Desorption Ionization/Time-of-Flight Mass Spectrometry (CAT#: STEM-ST-0334-LJX)
Analysis of organic environmental pollutants by surface-enhanced laser desorption/ionization mass spectrometry with polydopamine nanospheres as a substrate (CAT#: STEM-ST-0337-LJX)
Simultaneous detection of phosphatidylcholines and glycerolipids by matrix-enhanced surface-assisted laser desorption/ionization-mass spectrometry (CAT#: STEM-ST-0355-LJX)