Direct Detect Spectrometer, Millipore (CAT#: STEM-CTEPO-0091-ZJF)

The manufacturer of the Direct Detect Spectrometer, Millipore is Millipore.
Refurbished Millipore Direct Detect Spectrometer (p/n DDHW00010-WW) complete with direct detect spotting tray (p/n DDMISC0010-ST) and Dell laptop.
The Direct Detect™ infrared (IR)-based quantification system is an innovative combination of software-controlled instrumentation and EMD Millipore Corporation’s advanced membrane technology, optimized for the detection and quantification of proteins. Biomolecules are applied directly to a card-based hydrophilic polytetrafluoroethylene (PTFE) membrane that is transparent in most of the infrared spectral region.
The Direct Detect™ system measures amide bonds in protein chains, accurately quantifying an intrinsic component of every protein without relying on amino acid composition, dye binding properties, or redox potential. Protein concentrations from 0.2 to 5 mg/mL can accurately be determined from a minimal sample volume (2 μL) without bio- or immuno-chemical staining. Sample analysis takes only minutes and in most cases can be performed directly from the buffered or native solution.

Condition: Refurbished, Certified Pre-owned
Quantitation Approach, Assay Range, Sample Volume (tube/plate)
Direct Detect® System: 250-5,000 µg/mL, 2 µL
Bradford Assay: 20-2,000 µg/mL, 50 µL/25 µL
Micro BCA™ Assay: 2-4 µg/mL, 500 µL/150 µL
Lowry Assay: 10-1,500 µg/mL, 200 µL/40 µl

IR spectrometry measures amide bond absorbance for more reproducible quantitation across all proteins and peptides
Improves accuracy over traditional colorimetric assays
More compatible with detergents, reducing agents and other buffer components that interfere with traditional protein quantitation assays, providing more information from your sample
Preserves precious samples—requires only 2 μL per analysis
New software module can now quantify long aliphatic chains in lipids and detergents

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