Discrimination of Food-Borne Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (CAT#: STEM-ST-0319-LJX)

Introduction

A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

In a very small area and a very short time interval (ns order of magnitude), the laser delivers high-intensity pulse energy to the sample under test, causing it to desorption and ionize instantaneously without thermal decomposition. MALDI is a mass spectrometry ionization method for direct evaporation and ionization of non-volatile samples.

Applications

For measuring the molecular weight of biological macromolecules, such as the molecular weight distribution of peptides, proteins, nucleic acids, polymers and oligomer analysis.

Procedure

1. Mix the sample with the appropriate matrix material and load it onto the metal plate.
2. Pulsed laser light is used to irradiate the sample and trigger ablation and desorption of the sample and matrix materials.
3. Analyte molecules are ionized by protonation or deprotonation in the thermal plume of the ablated gas and are then accelerated to a mass analyzer for analysis.

Materials

• Sample Type:
Food-Borne microorganisms

Notes

1. During shutdown, if the nitrogen is not turned off, the pressure should be properly lowered to avoid moisture.
2. Keep an eye on instrument drift during manual measurement. If there is drift, the instrument needs to be calibrated with standard peptide or standard protein.

Other recommended products

Fast and Reliable Identification of Clinical Yeast Isolates by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (CAT#: STEM-ST-0305-LJX)
Direct Identification of Urinary Tract Pathogens from Urine Samples by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (CAT#: STEM-ST-0315-LJX)
Identification of Rare Pathogenic Bacteria in a Clinical Microbiology Laboratory by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (CAT#: STEM-ST-0309-LJX)
Analysis of protein single crystal by IR-matrix-assisted laser desorption/ionization (CAT#: STEM-ST-0307-LJX)
Oligosaccharides from Human Milk as Revealed by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (CAT#: STEM-ST-0323-LJX)
Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry (CAT#: STEM-ST-0312-LJX)
Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (CAT#: STEM-ST-0318-LJX)
Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (CAT#: STEM-ST-0297-LJX)