Display neurofibrils by Cajal silver staining technology (CAT#: STEM-CT-0009-LJX)

Silver staining, also known as silver plating staining, is one of the important staining methods for nerve tissue. The basic operating method is to first immerse the tissue slices with silver nitrate or mercury chloride to precipitate silver or mercury ions, phosphates, urates, etc. After washing, silver or mercury is reduced by formaldehyde, photographic developer, or sunlight, resulting in a black staining phase due to the precipitation of metallic silver or mercury. In this method, the reduction of silver or mercury salts is carried out by adding reducing agents or sunlight from outside the cell, while reducing substances derived from the cell are unrelated to this.

Staining of nerve tissue section samples from experimental animals

1.After fixing the tissue, soak it in pure water for a while.
2.Soak and fix the tissue in pyridine solution.
3.Soak the tissue in pure water.
4.Move the tissue into a 37℃ incubator and dye it in the dark with silver nitrate solution for 2 to 3 days.
5.Soak the tissue in pure water for a while.
6.Wash with ethanol.
7.Put the tissue into the color developing solution for color development.
8.Wash with pure water
9.The tissue was dehydrated by gradient ethanol, sliced by transparent xylene and embedded in paraffin for microscopic examination.

• Sample Type:
Nerve tissue section samples from experimental animals

If the sample is too lightly stained, it can be immersed in 0.2% gold chloride solution for color enhancement after dewaxing the section.