Display pyramidal cells in the brain by Golgi-Cox silver staining technology (CAT#: STEM-CT-0007-LJX)

Introduction

The silver staining method was first established by Italian physiologist Camillo Golgi in 1873, known as the Golgi's silver plating method or Golgi's silver staining method. Its basic principle is that silver ions of nitrate can combine with nerve fibrils in nerve cells to display the cytoskeleton and cell morphology of nerve cells, including neuronal soma, processes, nerve fibers and synapses.
In 1891, Cox made significant modifications to this method. He used a solution of mercury chloride and potassium dichromate mixed with potassium chromate to impregnate the sample and reduce the acidity of the solution to improve the color rendering effect, which was later known as the Golgi Cox method. This method is mainly used to show the appearance of cells but not their internal structure. It can also better show the pyramidal cells in the brain, the Purkinje cell in the cerebellum and their processes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Silver staining, also known as silver plating staining, is one of the important staining methods for nerve tissue. The basic operating method is to first immerse the tissue slices with silver nitrate or mercury chloride to precipitate silver or mercury ions, phosphates, urates, etc. After washing, silver or mercury is reduced by formaldehyde, photographic developer, or sunlight, resulting in a black staining phase due to the precipitation of metallic silver or mercury. In this method, the reduction of silver or mercury salts is carried out by adding reducing agents or sunlight from outside the cell, while reducing substances derived from the cell are unrelated to this.

Applications

Staining of nerve tissue section samples from experimental animals

Procedure

1.Rat perfusion fixation
2.The whole rat brain was placed in a wide brown bottle filled with Golgi-Cox dye
3.After sealing the bottle, place it in an anti-light container, and then place it in a 37℃ incubator for dip-staining.
4.After staining, remove the specimen and wash it with pure water for 3 times
5.Slice
6.Color with ammonia
7.Wash with pure water
8.Attach the section to the clean slide
9.Xylene transparent
10.Seal the slices

Materials

• Sample Type:
Nerve tissue section samples from experimental animals

Notes

1.The dye solution should be prepared for use and stored away from light.
2.The surface and bottom of the dye solution are prone to sediment, so it must be filtered before use.
3.During the slicing process, try to flatten the slices as much as possible to prevent them from breaking during the sealing process.

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Stain mouse brain tissue by Golgi-Cox silver staining technology (CAT#: STEM-CT-0008-LJX)