Endothelial surface glycocalyx (ESG) components and ultra-structure revealed by stochastic optical reconstruction microscopy (STORM) (CAT#: STEM-MIT-0385-LJX)

Introduction

In order to play different roles in vascular functions as a mechanosensor to blood flows and as a barrier to transvascular exchange, the endothelial surface glycocalyx (ESG) should have an organized structure. Due to the limitations of optical and electron microscopy, the ultra-structure of ESG has not been revealed until the recent development of super-resolution optical microscopy, STORM.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Principles of stochastic optical reconstruction microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Imaging in two or three dimensions, in multiple colors, and even in living cells
Applied in many areas of the life sciences, and provides very high resolution images for many different needs from neuroscience to subcellular science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Endothelial surface glycocalyx

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

Other recommended products

Revealing the nanoscale organization of pathological aggregates in human brain by stochastic optical reconstruction microscopy (CAT#: STEM-MIT-0376-LJX)
Investigation of Endothelial Surface Glycocalyx Components and Ultrastructure by Stochastic Optical Reconstruction Microscopy (STORM) (CAT#: STEM-MIT-0373-LJX)
Monitoring autophagy by super-resolution structured illumination and direct stochastic optical reconstruction microscopy (CAT#: STEM-MIT-0377-LJX)
Imaging of peroxisomes by stochastic optical reconstruction microscopy (CAT#: STEM-MIT-0372-LJX)
Imaging of Extracellular Vesicles in Three Dimensions by Direct Stochastic Optical Reconstruction Microscopy (CAT#: STEM-MIT-0374-LJX)
Monitoring the distribution of internalized silica nanoparticles inside cells by direct stochastic optical reconstruction microscopy (CAT#: STEM-MIT-0381-LJX)
High-efficiency localization of Na(+)-K(+) ATPases on the cytoplasmic side by direct stochastic optical reconstruction microscopy (CAT#: STEM-MIT-0390-LJX)
Volumetric super-resolution imaging of mouse neural tissue by serial ultrasectioning and stochastic optical reconstruction microscopy (CAT#: STEM-MIT-0386-LJX)