Endotoxin Detection (CAT#: STEM-MB-0127-WXH)

• Endotoxin detection not only helps to identify whether it is a bacterial infection, especially for Gram-negative bacilli infection, it can also indicate the severity of the infection, and can be used for rapid auxiliary diagnosis
• It is beneficial to clinical early diagnosis and treatment of diseases, provides a certain theoretical basis for the selection of drugs, and conducts dynamic monitoring of the disease, which is of great significance to the curative effect and prognosis judgment after drug administration.
• It is used for the monitoring of hemodialysis fluid, which plays an important role in ensuring the treatment effect and patient safety.

1. Limulus test method: Limulus test method is realized through a cascade reaction. Endotoxin activates factor C (FC) with the participation of divalent cations, and then activates other zymogens to produce a series of clotting enzyme reactions.
2. Recombinant factor C method: In the reaction, the recombinant factor C activated by endotoxin cleaves the fluorescent substrate to generate a fluorescent complex, which is quantified by quantitative detection of the fluorescent complex.
3. Pyrogen test method: This method is a relatively wide method for detecting pyrogens, and its content is tested by using the characteristic that a small amount of endotoxin can cause an increase in animal body temperature.
4. Other methods: immunological methods (enzyme-linked immunosorbent assay, rocket immunoelectrophoresis simple test, double antibody sandwich L mountain SA method, etc.), biological methods, chemiluminescence, flow cytometry, liquid chromatography, etc.