Endpoint PCR (CAT#: STEM-MB-0189-WXH)

Introduction

The polymerase chain reaction works by amplifying a segment of DNA bracketed by two inward-facing oligonucleotide primers in multiple cycles of template denaturation, primer hybridization and polymerization. In theory, the amount of DNA in the reaction doubles with every cycle, so it should be possible to deduce the amount of template DNA at the start of the reaction by how much is present at the end of reaction—a strategy called endpoint PCR. In the endpoint PCR, quantification is performed after the completion of the PCR reaction. Based on the total fluorescence emitted, the amount of template is determined into the sample. The method is also called as semi-quantitative PCR.