Enzyme Multiplied Immunoassay (EMIT) for qualitative and quantitative determination of proteins (CAT#: STEM-MB-0172-WXH)

Introduction

Enzyme multiplied immunoassay technique (EMIT) is a common method for qualitative and quantitative determination of therapeutic and recreational drugs and certain proteins in serum and urine.
It is an immunoassay in which a drug or metabolite in the sample competes with a drug/metabolite labelled with an enzyme, to bind to an antibody. The more drug there is in the sample, the more free enzyme there will be, and the increased enzyme activity causes a change in color.
In ELISA, the enzyme is a passive passenger through the actual immunoassay. In EMIT, the enzyme plays a key role throughout the assay process. ELISA requires very little knowledge of enzyme technology, whereas enzymology is the key to success in EMIT.