Eukaryotic membrane tethers by Magnetic tweezers (MT) (CAT#: STEM-MB-1252-WXH)

Introduction

Membrane tethers are long, thin tubes with walls constituted by lipid bilayers. They play important functional roles in eukaryotic cells and their organelles (such as the endoplasmic reticulum, mitochondria, and Golgi apparatus).

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Principle Applications Procedure Materials

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

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