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Evaluation of jejunal microvasculature of healthy anesthetized dogs by sidestream dark field video microscopy (CAT#: STEM-MIT-0349-LJX)

Introduction

Microcirculation is the blood circulation in the capillaries between tiny arteries and tiny veins. It is the beginning of lymphatic circulation and the most basic structural and functional unit in the circulatory system.
The side-flow dark field video microscopy technique can be used to evaluate jejunal microvascular circulation in healthy dogs.




Principle

The main difference between a dark field microscope and an ordinary microscope is the way of illumination. It illuminates the specimen with a strong, narrow, slanted beam without letting the beam enter the objective lens. When no light enters the objective lens, the field of view is dark, so it is called dark field microscope. However, because the particles in the specimen can scatter light after being illuminated by light, when the scattered light enters the objective lens, the scattered light spots of the particles can be seen in the microscope, as if the particles themselves were glowing. This is just as in a dark room, through a small hole in the wall of a strong beam of sunlight, we can see the presence of dust in the light path. This phenomenon, in optics, is known as the Dundar phenomenon. The dark-field microscope is designed according to this principle.

Applications

For observing microorganisms, colloid chemistry, single-celled organisms, and objects with linear structure
Unsuitable for observing stained specimens

Procedure

1. Sample preparation
2. Assembly and adjustment of dark field microscope
3. Observation

Materials

• Sample Type:
Dog jejunum

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures
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