Examination of fibre by Raman Spectroscopy (CAT#: STEM-ST-0027-WXH)

Introduction

Fibres are also one of the commonly encountered evidence during forensic investigation especially black/grey and blue cotton type fibres. The fibres are generally coloured by reactive dyes, as dyes can able to form non-polar bonds with fibres, which can produce difficulty in extraction during analysis. Raman spectroscopy measures the vibrational states of non-polar bonds through the use of high intensity lasers and can be used as analytical tool for fibre examination.

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Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

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